Phone: 352-372-3505
SUPERVISORY:
In charge of Nernatology Bureau from 1960-1967 and 1982-1984 (Biennium)
PROCESSING AND NEMATODE HANDLING
METHODOLOGY:
1) California can (introduced and tested in 1966)
2) Centrifuge method (introduced and tested in 1961)
3) Cyst processing procedures (original)
4) Devised a new en-face technique
5) Fenwick can (introduced and tested in 1960)
6) Foliar nematode diagnostic method (original)
7) Four minute fixation method (original)
8) Oostenbrink apparatus (introduced and tested in 1960)
9) Post Baermann funnel technique (original) 1958
10) Processing methods m1meo (1962) N-47
11) Seinhorst elutriator (introduced and used 1959-1960)
12) Steam Kettle and Pasta Sieve soil processing tandem (introduced 1987 and now in use)
(original)
13) Cover support technique (original)
14) Water agar cell technique 1957
15) Iodine fixation technique 1990
16) Baermann funnel holder
17) Pegboard apparatus supports
18) Soil sampling tool (Merit award) idea and co-inventer. Since its introduction in 1965, no
better tool has appeared. It has saved hundreds of sampling time hours by field personnel.
It has been adapted by IFAS, Georgia and Bermuda.
NEMATODE CODE:
Established 1960. A total of 223 genera and 5,263 species have been encoded as of 1991.
The entire code is in a computer mode for multi-purpose access. This code has saved
many hundreds of hours in Nematologist, technician, and secretarial time since its
inception.
EDUCATION:
1) Constructed burrowing nematode models, male and female (1958)
2) Lectures at University of Florida
Biology department (Invertebrate Zoology) since 1960 Nematology department
(Quarantine) since 1972
Soils department (Microbiology) since 1969
3) Promoted and headed up first field trilogy school (1961)
4) Wrote original trainee school course in its entirety
5) State Science Fair Judge (2 years)
6) Designed a comprehensive laboratory technician training program
7) Established first technician training manual
8) Slide talks prepared and permanently available:
A) Cyst nematode
B) Biological control
C) Burrowing nematode
D) Invertebrate zoology
E) Sanitation
F) Generalized : This talk has been duplicated and purchased by the following geographical
areas:
a) India
b) Netherlands
C) Pakistan
d) South Africa
e) USA: California
Florida
Georgia
SAFETY:
1) Organized first DPI safety committee (defunct)
2) Established eye wash kits in appropriate areas in DPI
3) Established chemical safety wall charts in appropriate areas of DPI
4) Instigated locking file cabinets to wall to prevent injury by double drawer open tip over
5) Established (by committee) guidelines for space fumigation safety
6) Devised eye injury emergency treatment chart
PUBLICATIONS:
A total of 197 publications
TAXON0MY:
1) Subfamily erected: Verutinae
2) Genera erected: Meloidodera, Verutus
3)Species described:
Meloidodera floridensis
Hemicriconemoides chitwoodi
Hemicriconemoides minutus
Hemicriconemoides strictathecatus
Verutus volvingentus
Xiphinema macrostylum
Tylenchulus graminus
Tylenchulus palustris
Merlinius paniculoides
Ogma floridensis
Criconemlla omicauda
Taxonomic compendia and keys have been devised for the following taxons and
categories:
A) Belonolaimus K) Heterodera
B) Bursaphelenchus L) Hoplolaimus
Q Criconerna M) Longidorus
D) Criconemoides N) Meloidogyne
E) Criconernatidnae 0) Monochidae
F) Discocriconemella P) Paratylenchus
G) Dolichodorus Q) Pratylenchus
H) Gracilacus R) Trichodorus
I) Hernicriconemoides S) Xiphinerna
J) Hemicycliophora
REGULATORY:
1) Turf regulations. Devised for Florida and Georgia in 1964
2) Organization of first "Society of Nernatologist" regulatory colloquium
3) Survey of North American regulatory nernatology (published results)
DATA RETRIEVAL REFERENCE FILES ESTABLISHED:
1) DPI reprint file by author
2) DPI taxonomic file by phytoparasite
3) DPI publications in nernatology
4) Control:
A) chemical (1960)
B) hot water ( 1 960)
5) Geographic distribution of nematodes (World) 1961
6) Host (I 959)
7) Map (I 980)
8) Natural classification (1978)
9) Photo (1959)
10) Phytoparasite ( 1 959)
11) Slide 2X2
12) Subject general (1962)
13) Taxonomic comprehensive (1969)
14) Taxonomic phytoparasite (1961)
SANITATION:
In 1965 a survey of sanitation in Florida nurseries resulting in establishment of a state wide
sanitation program.
1) A sanitation slide tale has been prepared which is used in all DPI training classes and
nursery
talks.
2) A large chart of sanitation was prepared that hangs in nurseries throughout Florida.
I consider sanitation one of my most productive accomplishements.
SURVEYS:
The following comprehensive surveys have been conducted in Florida. The principal
pest detected for the first time was pinewood nematode.
1) Biological control
2) Citrus grove
3) Aquatic plant
4) Corn
5) Cabbage (sugar beet nematode)
6) Pinewood nematode
7) Fire ant biological control agent survey
8) Grape survey
9) Sanitation survey
10) Lethal yellows survey
11) Golden nematode of potato
12) Honeybee: Biological control
13) Soil pit and compost
14) "Natural Area" University of Florida
A collection methods mimeo was prepared in 1958. A comprehensive survey manual
has been completed which will be hopefully printed in 1996.
COLLECTIONS:
1) Bottle specimens-Extensive
2) Bottle symptom specimens-Extensive
3) Stock identified specimens-Limited
4) Specimen slides-Extensive
COMPUTER:
1) Devised citrus nematode date retrieval program ( 1 98 1) (not implemented)
2) Devised Nernatology publication retrieval program (implemented)
3) Provided basic data that placed all species and genera of nematodes in Nernatology
Bureau computer in a coded and encoded format (implemented)
PHOTOGRAPHY:
Two innovations in photographic methodology have been developed and published
SCIENTIFIC ORGANIZATIONS:
1) Associate editor Society of Nernatologist (2 years)
2) Co-editor Nernatology Newsletter (Society of Nernatologist) (3 years)
3) Data retrieval committee, Society of Nernatologist (member and chairman)
4) Executive committee Society of Nernatologist (3 years)
5) Florida Nematology Forum co-organizer
6) Member Interscience Consortium For Plant Protection, representing the Societ
Nematologist (3 years)
7) Chairman Joint Task Force Group "Standard Methods for Examination of
Water"
8) Member Systematics Resources, Committee Society of Nematologist
FORMS DEVISED:
1) Diagnostic slips (1960) Exhibit 1
2) Field collection slips (1972) Exhibit 2
3) Laboratory analysis form (I 97 1) Exhibit 3
4) Pathological problem form (I 959) obsolete
5) Plant parasitic nematode list
(1961) Exhibit 4
6) NCR paper, for use with diagnostic slips (conceived by Esser and
Seymour) Exhibit 5
7) Numbered manuscript paper (merit award)
COMPOST INVESTIGATION:
1) Early in 1993, 1 joined a Compost Regulatory Task Force established by the
commissioner of agriculture.
2) Compiled compost data, conducted a National Compost Survey.
3) Gave two compost lectures; a) At a meeting in Orlando entitled "National Survey,
and a Southeastern meeting in Clearwater entitled "Composting Guidelines."
4) Published:
a) National Composting Survey Results - I lp.
b) "Composting Guidelines for Florida Composter - 22p.
EARTHWORM MALADY:
I
In 1960-61 disease appeared in Florida earthworm growing operations that wiped out
several worm farms and threatened the entire industry. I investigated the problem, coordinated
research and was instrumental in the formation of a worm farmers organization formed to
combat the disease. I accumulated a considerable amount of data concerning earthworms and
statewide earthworm problems have come to me since 196 1.
SEED AND GALL NEMATODE:
Completed a Seed and Gall nematode comprehensive synopsis for a computer
projected program in England.
SAMPLES DIAGNOSED: 124,000
BIOLOGICAL CONTROL:
Biological Control interactions between nematodes ad a variety of organisms that prey on
nematodes have been studied in and out of culture since 1962. Twenty-four biological control
publications have been produced and an illustrated guide to species of fungi attacking nematodes
prepared. A number of predacious organisms have been detected in Florida for the first time,
such as Pasteuria penetrans and Dactylella megalospora. P. penetrans has been the subject
of
considerable research at the University of Florida. Several predacious fungal cultures have been
deposited in the "American Type Culture Collection" in Maryland.
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